Deoxynivalenol （DON），also known as Vomitoxin，belongs to trichothecene and is produced by mould fusarium，growing in grains such as wheat，maize，barley and stover. Because of causing vomitting in porcines，hence the name is Vomitoxin. It has some harmful effects to human body and has been listed as Category III carcinogen by EU classification criteria. In 2011，the limit of Deoxynivalenol residues is 1000ug/kg in grains and grains products in China. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 15 minutes.Sample Type：Feed，soybean mealIntended Use：This BioAssay kit allows for the in vitro quantitative determination of Deoxynivalenol concentrations in feed，soybean meal.Sensitivity：1ug/kgRange：1-81ug/kgPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Deoxynivalenol antigen. Standards or samples are added to the appropriate microtiter plate wells with Deoxynivalenol antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Deoxynivalenol and Deoxynivalenol in standards or samples with the Deoxynivalenol antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Deoxynivalenol in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x50mlSample DIluent （48T），1x25mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
