Western blotting （1:500 – 1:2000） and Immunoprecipitation. However， the optimal concentrations should be determined individually. The antibody recognizes the NCL of human and mouse origins. HeLa and NIH3T3 cell lysates can be used as positive controls. Reactivity to other species has not been tested.
免疫原
Synthetic peptide of human NCL
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的Nucleolin蛋白发生免疫反应.
保存建议
提供的Nucleolin抗体产品为100 ul 无色溶液，其浓度为N/A，溶解于包含有30%甘油， 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Nucleolin抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
