TUCAN （Tumor Up-regulated Card-containing Antagonist of Caspase-Nine） is a CARD （Caspase-Associated Recruitment Domains） containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1，thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human， mouse， and rat origins. Jurkat cell lysate， mouse small intestine and rat kidney tissue lysates can be used as positive controls.
免疫原
Synthetic peptide surrounding amino acid 420 of human TUCAN
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的TUCAN/CARD8蛋白发生免疫反应.
保存建议
提供的TUCAN/CARD8抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5 BSA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该TUCAN/CARD8抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
