Sample Type：Serum，plasma，tissue homogenates and other biological fluids.Intended Use：Competitive ELISA，Coated with AntigenSensitivity：<0.094ng/mlRange：0.156-10ng/mlSpecificity：HumanTest Principle：This BioAssay ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Vitamin K. During the reaction，Vitamin K in the sample or standard competes with a fixed amount of Vitamin K on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Vitamin K. Excess conjugate and unbound sample or standard are washed from the plate，and HRP-Streptavidin （SABC） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Vitamin K in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components：Micro ELISA Plate （Dismountable），8x12Lyophilized Standard，2xvialSample/Standard dilution buffer，20mlBiotin-detection antibody （Concentrated），120ulAntibody dilution buffer，10mlHRP-Streptavidin Conjugate （SABC），120ulSABC dilution buffer，10mlTMB substrate，10mlStop solution，10mlWash buffer （25X），30mlPlate Sealer，5xpiecesProduct Description，1xcopyStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到Vitamin K BioAssay ELISA Kit （Human）产品后将其置于4°C至-20°C保存。
