Sample Type：Serum，plasma，tissue homogenates and other biological fluids.Intended Use：Sandwich ELISA，Double AntibodySensitivity：<0.281ng/mlRange：0.469-30ng/mlSpecificity：HumanTest Principle：This BioAssay kit was based on sandwich enzyme-linked immune-sorbent assay technology. Visfatin C termina antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Visfatin C termina antibody was used as detection antibodies. The standards，test samples and biotin conjugated detection antibody were added to the wells subsequently，and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Visfatin C termina amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader，and then the concentration of Visfatin C termina can be calculated.Kit Components：Micro ELISA Plate （Dismountable），8x12Lyophilized Standard，2xvialSample/Standard dilution buffer，20mlBiotin-detection antibody （Concentrated），120ulAntibody dilution buffer，10mlHRP-Streptavidin Conjugate （SABC），120ulSABC dilution buffer，10mlTMB substrate，10mlStop solution，10mlWash buffer （25X），30mlPlate Sealer，5xpiecesProduct Description，1xcopyStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
