The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines （such as TNF- and IL-1），UV light，inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 （also known as MKK4），which in turn activates SAPK （also known as JNK）. JNK binds tightly to the N-terminal region of c-Jun and ATF-2，and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
应用类型
Western blotting （0.5-4 ug/ml），immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. Reacts with human and to a lesser extent with mouse and rat. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 412 of human JNK-1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的JNK蛋白发生免疫反应.
保存建议
提供的JNK抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该JNK抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
