XAF-1 binds to XIAP，an inhibitor of caspases-3，-7，-9 and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death. XAF-1is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast，XIAP levels have been shown to be high in a majority of cell lines.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2401-100） can be used as the positive control. The antibody recognizes 34 kDa XAF-1 from samples of human origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding a.a. 306 of human XAF-1
来源宿主
兔
反应性
经检测验证，可与人种属来源的XAF-1蛋白发生免疫反应.
保存建议
提供的XAF-1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该XAF-1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
