HNF-4 （Hepatocyte nuclear factor 4），a member of the steroid/thyroid nuclear receptor superfamily，is a transcriptional factor which expresses in the liver，intestine，kidney，and pancreatic cells. It contains several functional domains: a ligand-independent activation domain （AF1），a zinc finger DNA binding domain，and a ligand-dependent activation domain （AF2）. HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes，involved in glucose，fatty acid，and cholesterol metabolisms.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Mouse small intestine and rat kidney （Cat.# 2702-100） tissue lysates can be used as positive controls.
免疫原
Synthetic peptide surrounding amino acid 454 of rat HNF
来源宿主
兔
反应性
经检测验证，可与小鼠、大鼠种属来源的HNF-4蛋白发生免疫反应.
保存建议
提供的HNF-4抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该HNF-4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
