JIP1 （JNK-interacting protein-1） is a cytoplasmic protein that possesses an N-terminal JNK-binding domain and a C-terminal SRC homology 3 （SH3） domain. JIP1 selectively bind to JNK，but not to the ERK or p38 MAP kinases. In addition to JNK，JIP1 binds to specific proteins of JNK pathways，including MKK7，MLK3，and DLK，all at separate binding sites. JIP1 has been identified in brain and kidney tissues，whereas JIP2a，JIP2b，and JIP3 are specifically expressed in the brain. Overexpression of JIP1 causes cytoplamic retention of JNK，thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathways，indicating that JIP1 acts as a cytoplasmic anchor for JNK.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 78 kDa JIP1 in samples from mouse， rat and human origins. In human sample， a ~110 kDa isoform can also be observed. Reactivity to other species has not been tested.
免疫原
Synthetic peptide from mouse JIP1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的JIP-1蛋白发生免疫反应.
保存建议
提供的JIP-1抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS缓冲液中.请将该JIP-1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
