MARCKS （Myristoylated alanine-rich protein kinase C substrate） is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility，cell adhesion，phagocytosis，membrane traffic and mitogenesis. PKC phosphorylates Ser152，156 and 163 of MARCKS，which regulates MARCKS’s calcium/calmodulin binding activity and filamentous （F）-actin cross-linking activity. In addition，phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
应用类型
Western blotting （1:200-1000）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.
免疫原
Synthetic peptide corresponding to amino acids surrounding Ser152/156 of rat MARCKS
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Phospho-MARCKS蛋白发生免疫反应.
保存建议
提供的Phospho-MARCKS抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Phospho-MARCKS抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
