Cytochrome c oxidase （COX） is a 13-subunit complex spanning the inner mitochondrial membrane and responsible for the terminal reduction of dioxygen to water in the electron transport chain. The three core catalytic units COX-1，-2，-3 are trans-membrane proteins encoded by the mitochondrial genome，whereas the remaining 10 subunits are nuclear encoded and expressed in a tissue-specific manner. The expression of nuclear and mitochondrial subunits of the mitochondrial respiratory chain is thought to be highly coordinated. Cox-4 is believed to regulate COX activity according to the extramitochondrial ATP/ADP ratio.
应用类型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 32 of human Cox IV
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Cox-4蛋白发生免疫反应.
保存建议
提供的Cox-4抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Cox-4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
