Selectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naïve T cells and on most monocytes,neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells,monocytes,and neutrophils.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes L-Selectin in human sample, and in a lesser extent to mouse, and rat samples.
免疫原
Synthetic peptide surrounding amino acid 69 of human L-Selectin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的L-Selectin/CD62L蛋白发生免疫反应.
保存建议
提供的L-Selectin/CD62L抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该L-Selectin/CD62L抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.