The nucleosome is made up of four core histone proteins （H2A，H2B，H3 and H4） and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation，phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
应用类型
Western blotting （0.5-4 ug/ml）， in immunoprecipitation （20-40 ug/ml） and Immunohistochemistry （20-40 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~11 kDa histone H4 protein. It does not cross-react with other histones.
免疫原
Synthetic peptide corresponding to the N-terminus of human Histone H4
