Human HDAC6 （1215 a.a. residues） possesses two separate putative catalytic domains，both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6，which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.
应用类型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins including HDAC1， 2， 3， 4， 5， 7， and 8.
免疫原
Synthetic peptide mapping to the N-terminus of mouse HDAC-6
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的HDAC6蛋白发生免疫反应.
保存建议
提供的HDAC6抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该HDAC6抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
