Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
应用类型
Western blotting （0.5-4 ug/ml）， Immunoprecipitation （20 ug/ml）， and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~124 kDa HDAC5 with human and mouse samples， and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins including HDAC1， 2， 3， 4， 6， 7， 8， 9，10， and 11.
免疫原
Synthetic peptide surrounding amino acid 575 of human HDAC-5
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的HDAC5蛋白发生免疫反应.
保存建议
提供的HDAC5抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该HDAC5抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
