HDAC4 is a member of the class II mammalian histone deacetylases，which consists of 1084 amino acid residues. Its C-terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4，unlike other deacetylases，shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus，HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~140 kDa human HDAC-4. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 10 of human HDAC-4
来源宿主
兔
反应性
经检测验证，可与人、鸡种属来源的HDAC4蛋白发生免疫反应.
保存建议
提供的HDAC4抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该HDAC4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
