Galectins are a family of soluble B-galactoside-binding animal lectins that modulate cell-to-cell and cell-to-ECM interactions,as well as apoptosis. Galectin-3 encodes a 30-35 kDa protein containing a single carbohydrate binding domain,which binds galactose-containing glycoconjugates. Upregulated expression of Galectin-3 is involved in cancer progression and metastasis.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes the 30 kDa human Galectin-3 in Western blot analysis with Jurkat cell lysate.
免疫原
Synthetic peptide surrounding amino acid 188 of human Galectin-3
来源宿主
兔
反应性
经检测验证,可与人种属来源的Galectin-3蛋白发生免疫反应.
保存建议
提供的Galectin-3抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Galectin-3抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.