The sCD14 （Soluble Cluster of Differentiation14） BioAssay ELISA Kit is a quantitative sandwich assay for the detection of sCD14 in human serum，plasma，tissue homogenates and other biological fluidsRange：0.313-20ng/mlSensitivity：0.188ng/mlPrecision： Intra-Assay CV： <8%Inter-Assay CV： <10%Kit Components：*358701A： Microtiter Strips，1x96 wells （8x12 wells）.*358701B： Standard，2x1 vial358701C： Sample/Standard Dilution Buffer，1x20ml358701D： Antibody （Biotin） （Concentrated），1x120ul358701E： Antibody Dilution Buffer，1x10ml358701F： Streptavidin （HRP） （SABC），1x120ul358701G： SABC Dilution Buffer，1x10ml358701H： TMB Substrate，1x10ml358701J： Stop Solution，1x10ml358701K： Wash Buffer，25X，1x30mlStorage and Stability：Store unopened *358701A at 4ºC; store at -20ºC once opened. Store unopened *358701B at 4°C; once reconstituted，store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Summary：1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody （Biotin） working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
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保存建议
建议收到sCD14 （Soluble Cluster of Differentiation14） BioAssay ELISA Kit （Human）产品后将其置于4°C至-20°C保存。
