Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT，a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~52 kDa Oct-2 in samples from human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide mapping to the c-terminus of human Oct-2
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Oct-2蛋白发生免疫反应.
保存建议
提供的Oct-2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Oct-2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
