ROCK-1 （Rho-associated coiled coil-containing protein kinase 1） has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis，ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed，resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing，the early events in the execution phase of apoptosis.
应用类型
Western blotting （1-4 ug/ml）. However， the optimal conditions should be determined individually. Jurkat cell lysate treated with camptothecin （2 uM for 6-8 hours） can be used as a positive control. The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.
免疫原
Synthetic peptide corresponding to the caspase-3 cleavage site
