Syntaxins were originally thought to be docking proteins，but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis，including VAMPs （vesicle-associated membrane proteins），NSF （N-ethylmaleimidesensitive factor），SNAP 25 （synaptosomal-associated protein of 25kDa），SNAPs （soluble NSF attachment proteins） and synaptotagmin. SNAPs mediate the membrane binding of NSF，which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.
应用类型
Western blotting （1-2 ug/ml）， immunoprecipitation （5-15 mg/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 85 kDa NSF of mouse， and rat origins. Reactivity to other species has not been tested
免疫原
Synthetic peptide corresponding to amino acids around 731 of rat NSF
来源宿主
兔
反应性
经检测验证，可与小鼠、大鼠种属来源的NSF蛋白 发生免疫反应.
保存建议
提供的NSF抗体 产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 叠氮钠的PBS缓冲液中.请将该NSF抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
