- 中文名称
C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)
- 英文名字
- C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 351744
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This Human CRP ELISA kit is to be used for the in vitro quantitative determination of human C-Reactive Protein (CRP) concentrations in serum. It is intended for research use only and not for use in diagnostic procedures.Principle:This CRP enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. CRP, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound CRP and other components of sample.In order to quantitate the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated antibody specific for CRP is added to each well to "sandwich" the CRP immobilized during the second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of an acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm±2nm. In order to measure the concentration of CRP in the samples, this kit standard (ready-to use) is assayed at the same time as the samples (diluted if necessary with Sample Diluent). This allows the operator to produce a standard curve of Optical Density (O.D.) versus CRP concentration (mg/L). The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: 0.03mg/LDetection Range: 0.005 -0.100mg/LKit Components: 351744A: Microtiter Plate, 1x96wells, Pre-coated with a monoclonal anti-human CRP351744B: CRP Ab (HRP), 1x12ml. Anti-human CRP labeled with HRP351744C: Standard 1, 0mg/L, 1x1ml. Buffered protein base with preservative.351744D: Standard 2, 0.005mg/L, 1x1ml. CRP in buffered protein base with preservative.351744E: Standard 3, 0.010mg/L, 1x1ml. CRP in buffered protein base with preservative.351744F: Standard 4 , 0.025mg/L, 1x1ml. CRP in buffered protein base with preservative.351744G: Standard 5, 0.050mg/L, 1x1ml. CRP in buffered protein base with preservative.351744H: Standard 6, 0.100mg/L, 1x1ml. CRP in buffered protein base with preservative.351744J : TMB Substrate, 1x11ml. Ready to use.351744K: Stop Solution, 1x11ml. 2N HCl. Caution: Caustic Material!351744L: CRP Sample Diluent, 1x50ml. PBS buffer with BSA as preservative.Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Materials Required but not Supplied:1. Single or multi-channel precision pipettes with disposable tips: 5-10ul, 100ul and 500ul 2. Multi-channel pipette reservoir or equivalent reagent container3. Distilled or deionized water4. Test tubes and racks5. Polypropylene tubes or containers (25ml)6. Erlenmeyer flasks: 100ml, 400ml, 1L and 2L7. Microtiter plate reader (450nm±2nm)8. Automatic microtiter plate washer or squirt bottle9. Sodium hypochlorite solution, 5.25% (household liquid bleach)10. Deionized or distilled water11. Plastic plate cover12. Disposable gloves13. Absorbent paperProtocol:1. Add 10ul of Standard or Sample to the appropriate well of the antibody pre-coated Microtiter Plate. 2. Dispense 100ul of conjugate to each well. Thoroughly mix for 30-60 seconds. 3. Cover plate and incubate for 45 minutes at room Ttemperature (18-25°C). 4. Wash the Microtiter Plate five times using 1X Wash Buffer, using one of the specified methods below:Manual Washing: Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with distilled or deionized water, then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure four more times for a total of 5 washes. After final wash, invert plate and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate firmly when washing the plate to assure that all strips remain securely in frame.Automated Washing: Aspirate all wells, then wash plates 5 times using distilled or deionized water. Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350ul/well/wash (range: 350-400ul). After final wash, invert plate and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. It is recommended that the washer be set for a soaking time of 10 seconds or shaking time of 5 seconds between washes.5. Add 100ul TMB Substrate to each well. Cover andiIncubate for 10-15 minutes at room temperature.6. Add 100ul Stop Solution to each well. Mix well.7. Read the Optical Density (O.D.) at 450nm using a microtiter plate reader within 30 minutes.
- 产品特点
- 针对C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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