c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity，and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity，but mutation of Ser621 to alanine results in an inactive kinase.
应用类型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （20-40ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
免疫原
Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf
