The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation，differentiation and cancer regulation. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by MAP Kinase at Ser62 and in vivo c-Myc is phosphorylated at both Thr58 and Ser62. Mutation of The58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription，suggesting that phosphorylation regulates the ability of c-Myc to stimulate transcription. c-Myc appears to regulate multiple aspects of growth control: it promotes cell proliferation in the presence of cdk inhibitors and induces apoptosis with overexpression in serum deprived cells.
应用类型
Western blot analysis （0.5-4 ug/ml）， immunoprecipitation （2-4 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The Phospho-c-Myc （Thr58/Ser62） antibody detects only phosphorylated Thr58/Ser62 of c-Myc. It does not react with non-phosphorylated c-Myc.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr58/Ser62 of human c-Myc
