Heat shock proteins （HSPs） are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly，HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1，serpinh1，collagen-binding protein 1 （CBP1） and gp46. The human HSP 47 gene encodes a 47 kDa protein.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml） and Immunohistochemistry （10-20 ug/ml） in frozen or paraffin embedded tissues. However， the optimal concentrations should be determined individually. The antibody recognizes 47 kDa Hsp47 of human， mouse， rat， and chick origins. Mouse small intestine tissue lysate （Cat.# 2601-100） can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 407 of human HSP47
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、鸡种属来源的Hsp47蛋白 发生免疫反应.
保存建议
提供的Hsp47抗体 产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Hsp47抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
