Methylation of DNA at cytosine residues plays an important role in regulation of gene expression，genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date，3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1，Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA （cytosine-5）-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
应用类型
Western blotting （0.5-4 ug/ml） and immunofluorescence. However， the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.
免疫原
Synthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的Dnmt2蛋白发生免疫反应.
保存建议
提供的Dnmt2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Dnmt2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
