Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH,O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide,iron and biliverdin that is immediately reduced to bilirubin. To date,three heme oxygenase isoforms HO-1,HO-2 and HO-3 have been identified.
应用类型
Western blotting (0.5-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~32-35 kDa Heme-Oxygenase-1. Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid near the N-terminus of human HO-1