D4-GDI （GDP dissociation inhibitor） is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death，the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase，a regulator of apoptosis，may be one of the mechanisms.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide near the N-terminus of human D4-GDI
来源宿主
兔
反应性
经检测验证，可与人种属来源的D4-GDI蛋白发生免疫反应.
保存建议
提供的D4-GDI抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该D4-GDI抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
