NF-kB is silenced in the cytoplasm by an inhibitory protein，IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins，IKK1/，IKK2/B，and IKK3/g. External stimuli such TNF- or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkB.
应用类型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （1-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects both phosphorylated and non-phosphorylated forms of IkB.
免疫原
Recombinant human IkBa
来源宿主
小鼠
反应性
经检测验证，可与人种属来源的IkBa蛋白发生免疫反应.
保存建议
提供的IkBa抗体产品为100 ug 无色溶液，其浓度为0.25 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该IkBa抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
