Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways，in which the key apoptotic proteases caspase-8 and caspase-9，respectively，are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER，but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress，including disruption of ER calcium hemeostasis，and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer’s disease including gamma-secretase presenilin and B-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-B. Caspase-12 is ubiquitously expressed in mouse tissues.
应用类型
Western blot
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
