The MBD family of proteins includes MeCP2，MBD1，MBD2，MBD3 and MBD4. MBD1 （methyl-CpG binding domain protein 1） binds to methylated CpG sites and represses gene transcription. MBD1 is recruited to both methylated and non-methylated CpGs by separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. MBD1 may also interact with histone methyltransferase SETDB1 to repress transcription.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Cross activity to perforin of other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 594 of human MBD1
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的MBD1蛋白发生免疫反应
保存建议
提供的100 ug MBD1抗体产品为无色溶液其浓度为0.5 mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该MBD1抗体产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
