AMPK beta-1 chain is a regulatory subunit of the AMP-activated protein kinase （AMPK）. AMPK is a heterotrimer consisting of an alpha catalytic subunit，and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses，AMPK is activated，and thus phosphorylates and inactivates acetyl-CoA carboxylase （ACC） and beta-hydroxy beta-methylglutaryl-CoA reductase （HMGCR），key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
应用类型
The antibody can be used for ELISA （0.25 ug/ml）， Western blotting （0.5 – 2.5 ug/ml） and Immunohistochemistry （2.5-5.0 ug/ml）.
免疫原
Synthetic peptide selected from the N-terminal region of human PRKAB1
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的AMPKB蛋白发生免疫反应.
保存建议
提供的AMPKB抗体产品为100 ug 无色溶液，其浓度为0.25 mg/ml，溶解于包含有0.09% （W/V） 叠氮钠的PBS缓冲液中.请将该AMPKB抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
