Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily throμgh interaction with their death domain (DD) containing receptors，TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1，Apo3，TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1，overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD，TRAF2，and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis throμgh TRADD，FADD，and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL)，thymus and spleen.
应用类型
Western blot and Immunohistochemistry
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 378 of mouse DR3
