Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis，cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit （35 kDa or 17-25 kDa） and small （10 kDa） by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3，one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage，procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 22 kDa band， corresponding to the expected molecular mass of RAIDD on immunoblots.
免疫原
Synthetic peptide surrounding amino acid 171 of human RAIDD
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的RAIDD蛋白发生免疫反应.
保存建议
提供的RAIDD抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该RAIDD抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
