Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1，or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-B1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells，Mcl-1 interacts with another Bcl-2-related protein，Bax，and prolongs cell viability after treatment with different apoptotic reagents.
应用类型
Western blot, Immunoprecipitation, Immunohistochemistry
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
