Caspases play a key role in apoptosis. Similar as other caspases，caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis，while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.
应用类型
Western blotting （1-2 ug/ml）. However， the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples， it also detects a 30 kDa intermeadiate fragment
免疫原
Synthetic peptide surrounding amino acid 417 of mouse caspase-2
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Caspase-2蛋白发生免疫反应.
保存建议
提供的Caspase-2抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Caspase-2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
