Cytochrome c （m.w. 12，500） is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis，prior to caspase activation，DNA fragmentation，and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.
