PARP，a 116 kDa nuclear poly （ADP-ribose） polymerase，is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215，which separates PARP’s N-terminal DNA binding domain （24 kDa） from its C-terminal catalytic domain （89 kDa）. It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
应用类型
Immunocytochemistry : 5-20 ug/ml； Western Blot （10 ug/ml using colorimetric methods； <2 ug/ml for ECL）； Immunoprecipitation and ELISA
免疫原
Poly（ADP-ribose） mixed with methylated bovine serum albumin
来源宿主
小鼠
反应性
经检测验证，可与哺乳动物种属来源的PARP蛋白发生免疫反应
保存建议
提供的100 ug PARP抗体（克隆号10H）产品为无色溶液其浓度为1.0 mg/ml溶解于包含有1% BSA、0.02%叠氮化钠和150 mM NaCl的20 mM Tris （pH 7.4）缓冲液中.请将该PARP抗体（克隆号10H）产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
