KLH antibody is used to detect Keyhole Limpet Hemocyanin in ELISA assays. KLH is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
应用类型
ELISA,Immunohistochemistry,Western Blot,
免疫原
Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
来源宿主
Mouse
反应性
Keyhole limpet hemocyanin (KLH)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
