- 中文名称
【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S
- 英文名字
- 【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S
- 供应商
- Rockland
- 产品货号
- 200-301-382S
- 产品报价
- ¥询价/25uL

- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻

- 背景资料
- 6X His antibody is an Epitope tag which is a short peptide sequence that is easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- 应用类型
- ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
- 免疫原
- This His antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
- 来源宿主
- Mouse
- 反应性
- 该【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S的详细信息查看6X His antibody is an Epitope tag which is a short peptide sequence that is easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.提供的产品说明书。
- 保存建议
- Store 6X His Tag Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 其他
- Rockland成立于1962年,是一家老牌抗体原产企业,其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体,包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素(Ubiquitin)等识别抗体,应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外,Rockland也生产特别齐全的二抗,包括纯化、生物素、HRP/ALP、荧光标记二抗,应用十分广泛。

- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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