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中文名称

Dihydro-Testosterone BioAssay ELISA Kit

英文名字
Dihydro-Testosterone BioAssay ELISA Kit
供应商
USBiological
产品货号
167550
产品报价
¥1/96Tests
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
5a-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity.The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Intended Use:The Dihydro- Testosterone ELISA kis is for the direct quantitative determination of Dihydrotestosterone by enzyme immunoassay in human serum. For in vitro diagnostic use only.Principle of the Test:The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in patient samples and controls can be directly read.Detection Range: 0-2500 pg/mlSpecificity: 100%Sensitivity: 6pg/mlKit Components:Microtiter Plate, 1x96wellsDihydrotestosterone-Horseradish Peroxidase (HRP) Conjugate Concentrate, 1x200ulCalibrator A 0pg/ml, 1x2mlCalibrator B 25pg/ml, 1x600ulCalibrator C 100pg/ml, 1x600ulCalibrator D 500pg/ml, 1x600ulCalibrator E 1000pg/ml, 1x600ulCalibrator F 2500pg/ml, 1x600ulControl, 1x600ulWash Buffer Concentrate, 1x50mlAssay Buffer, 1x15mlTMB Substrate, 1x16mlStop Solution, 1x6mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Dihydro-Testosterone BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Dihydro-Testosterone BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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