中文名称

【Caspase 3 Pro】重组小鼠Caspase-3前体蛋白（5 ug）

英文名字

Caspase-3 Proform, mouse recombinant

供应商

Biovision

产品货号

1183P-5

产品报价

￥已停产

产品说明书

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产品新闻

背景资料

Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or “effector” enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and –10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP “apoptosome”. Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c.

The recombinant Procaspase-3 (1183P-5) is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8 (1088-25, -100). We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100 ul of Reaction Buffer (1068-20) for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits (K105 and K106).

序列

重组小鼠Caspase-3前体蛋白蛋白产品，又称Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, FLICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1.其对应NCBI基因号为12367，NCBI基因别称为CASP3；该基因种属来源为小鼠源，对应Accession号为Q8BNT4.该蛋白产品又称Mouse Procaspase-3, Procaspase-3, procaspases, caspase-3 proenzyme, caspase-3 proform等.

来源宿主

提供的【Caspase 3 Pro】重组小鼠Caspase-3前体蛋白产品的来源为大肠杆菌中重组表达.

溶解建议

提供的重组小鼠Caspase-3前体蛋白 产品为冻干粉末建议将其重新溶解于包含有15%甘油的PBS缓冲液中至浓度为0.1 ug/ul.

保存建议

在您收到艾美捷发出的【Caspase 3 Pro】重组小鼠Caspase-3前体蛋白产品后，建议将其置于-70°C保存.

其他

BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的Leader，BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供优质、新的细胞生物学研究工具而不断扩大产品及服务质量.

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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