Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation. In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation. Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates. Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression. Troponin I Antibody is ideal for researchers interested in cancer research.
应用类型
ELISA,Western Blot,
免疫原
Anti-Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a fusion protein of complete mouse cardiac troponin 1.
来源宿主
Rabbit
反应性
Mus musculus (Mouse); Rattus (Rat)
保存建议
Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut
