【干冰】Anti-Erk2 (RABBIT) Antibody - 100-401-218-【干冰】Anti-Erk2 (RABBIT) Antibody - 100-401-218代理/参数/厂家-一抗-艾美捷科技有限公司

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背景资料: Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.

免疫原: Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.

保存建议: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。