- 中文名称
Adrenocorticotropic Hormone (ACTH) BioAssay ELISA Kit (Rat)
- 英文名字
- Adrenocorticotropic Hormone (ACTH) BioAssay ELISA Kit (Rat)
- 供应商
- USBiological
- 产品货号
- 023173
- 产品报价
- ￥1/48Tests ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Adrenocorticotropic Hormone (ACTH) BioAssay ELISA Kit (Rat) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ACTH in rat serum, plasma and other biological fluids.Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. A deficiency of ACTH is a cause of secondary adrenal insufficiency and an excess of it is a cause of Cushing's disease.Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241aa residues. It is synthesized by corticotrope cells of the anterior pituitary gland, melanotrope cells of the intermediate lobe of the pituitary gland, about 3000 neurons in the arcuate nucleus of the hypothalamus, and smaller populations of neurons in the dorsomedial hypothalamus and brainstem. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: adrenocorticotrophic hormone (ACTH) and gamma LPH in the anterior pituitary gland CLIP, gamma LPH, gamma MSH and beta-endorphin in the intermediate lobe gamma-MSH met-enkephalin. Each of these peptides is packaged in large dense-core vesicles that are released from the cells by exocytosis in response to appropriate stimulation. gamma MSH produced by neurons in the arcuate nucleus has important roles in the regulation of appetite and sexual behavior, while alpha-MSH secreted from the intermediate lobe of the pituitary regulates the production of melanin. ACTH is a peptide hormone that regulates the secretion of glucocorticoids from the adrenal cortex. beta-endorphin and met-enkephalin are endogenous opioid peptides with widespread actions in the brain.Detection Range:12.35-1000pg/mlSensitivity:4.39pg/mlIntra-Assay CV: <10%Inter-Assay CV: <12%Kit Components:*023173A: Microtiter Plate, 1x96wells, Pre-coated, ready to use*023173B: Standard, 2x1vial 023173C: Standard Diluent, 1x20ml*023173D: Detection Reagent A (green), 1x120ul *023173E: Detection Reagent B (red), 1x120ul 023173F: Assay Diluent A, 1x12ml023173G: Assay Diluent B, 1x12ml023173H: TMB Substrate, 1x9ml023173K: Stop Solution, 1x6ml023173L: Wash Buffer, 30X, 1x20ml Precaution:The Stop Solution (023173K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *023173A, *023173B, *023173D and *023173E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard, control or sample to each well. 3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul 023173H: TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul 023173K: Stop Solution. Read at 450nm immediately.
- 产品特点
- 针对Adrenocorticotropic Hormone (ACTH) BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Adrenocorticotropic Hormone (ACTH) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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