Human Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. Human LIF signals through gp130 to activiate STAT3 in human ESCs . In mouse ESCs, LIF promotes self-renewal and pluripotentcy, but in human cells there is no indication that LIF has the same function. Instead, FGF-basic (FGF-2) and Activin A are used to promote self-renewal and pluripotent potential. Recombinant human LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 19.9 kDa.
序列
该Human Leukemia Inhibitory Factor Recombinant Protein (Animal Free) - 009-F01-V72-0025的详细信息查看Rockland提供的产品说明书。
来源宿主
Other - E.coli
溶解建议
Restore with deionized water (or equivalent)
保存建议
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
