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OxiSelec总抗氧化能力(TAC)分析试剂盒

发布者:艾美捷科技    发布时间:2021-07-01     
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活性氧.png

 

活性氧(Reactiveoxygenspecies,ROS)主要包括羟基自由基、超氧自由基和过氧化氢。在细胞或组织的正常生理代谢过程中会产生活性氧,同时一些环境因子例如紫外照射、吸烟、环境污染等也可以诱导活性氧的产生。活性氧在体内的失衡,将会导致细胞脂、蛋白和DNA的氧化损伤,继而导致各种肿瘤、风湿性关节炎、糖尿病、肝损伤、以及中枢神经系统疾病等。

 

当然,机体中也存在许多种抗氧化的物质,包括抗氧化大分子、抗氧化小分子和酶等,能够清除体内产生的各种活性氧,以阻止活性氧造成的氧化损伤。一个体系内的各种抗氧化大分子、抗氧化小分子和酶的总的水平即体现了该体系内的总抗氧化能力。因此测定样本中的总抗氧化能力(TAC)具有非常重要的生物学意义。

 

作为专业的生命科学医药原料供应商,Cell Biolabs中国区金牌代理,艾美捷科技为您推荐:适用于组织裂解液,细胞裂解液,血清,血浆(柠檬酸盐  ),尿液,食物提取物的,OxiSelect 总抗氧化能力(TAC)分析试剂盒

 

试剂盒原理图.png

Fig. 试剂盒原理图

 

产品总抗氧化能力(TAC)检测试剂盒
Total Antioxidant Capacity (TAC) Assay Kit
货号STA-360 (200 assays)
检测方法比色法
样本类型组织裂解液,细胞裂解液,血清,血浆(柠檬酸盐 ),尿液,食物提取物
说明书下载点击下载
实验原理总抗氧化能力(TAC)检测试剂盒通过单电子转移(SET)机制测量来自各种样品的生物分子的总抗氧化能力。TAC检测基于抗氧化剂(如尿酸)将铜(II)还原为铜(I)。还原后,铜(I)离子进一步与偶联显色试剂反应,产生在490nm处具有最大吸光度的颜色。抗氧化剂的净吸光度值与已知的尿酸标准曲线进行比较。吸光度值与样品的总还原能力成正比。结果表示为“μM铜还原当量”或“mM尿酸当量”。铜比基于铁的抗氧化剂测定更有优势,因为所有类别的抗氧化剂,包括硫醇,都可以在边缘自由基干扰下进行检测。此外,铜基反应的动力学也比铁基反应快,这使得TACAssay成为高效准确估计还原或抗氧化能力的理想工具
试剂盒组分1. Uric Acid Standard
2. Reaction Buffer (100X)
3. Copper Ion Reagent
4. Stop Solution (10X)
保存条件收到后,将反应缓冲液(100X)保存在4?C下,将其它剩余的组分保存在室温下。

* 本产品仅适用于科研用途.

 

FAQ常见问题:

 

1.总抗氧化能力(TAC)检测试剂盒,有物种种属限制吗?

我们的总抗氧化能力(TAC)检测试剂盒,适用于任何生物蛋白质样品。因为试剂盒原理并不依赖于可能因物种而异的蛋白质结构。

 

2.是否可以检测冻存过的样本?

我们的总抗氧化能力(TAC)检测试剂盒,通常适用于在 -80?C 下保存两个月以内样品,但我们建议尽可能测试新鲜样品以获得最佳结果。

 

3.在制备食品样品时,1:2, w/v 是什么意思?

食品样品应准备为 1 g 样品:2 mL 去离子水。

 

4.柠檬酸盐血浆样品可以用于该测定吗?

柠檬酸盐血浆样品与总抗氧化能力测定兼容。 但应避免使用EDTA,因为这会干扰检测。

 

5.该试剂盒可以测量细胞培养上清液的总抗氧化能力吗?

不幸的是,我们的总抗氧化能力测定与细胞培养上清液不兼容。该测定基于铜的还原,介质中存在的铁会干扰该反应。

 

结果展示:

试剂盒标准曲线.jpg

试剂盒标准曲线

 

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