想象一下,当你回到家坐在沙发上, 看到茶几上有一块鲜红多汁的西瓜,于是你拿起西瓜大块朵颐。事实上,这个简单的场景包含了无数神经突触的协助。
我们都知道,神经信息的传递是靠一个神经元传递给下一个神经元的方式完成的。当视觉神经元接收到西瓜的信号后,神经元的轴突末梢,也就是突触小体,会与多个神经元的细胞体或树突相接触,形成突触,这种突触是由突触前膜、突触间隙和突触后膜三部分构成。突触前神经元的轴突顶枝释放突触囊泡,其内含物就是神经递质,神经递质将信号传递给下一个神经元的细胞体或树突,完成信号的单向传递;最后到达大脑,接收到神经元传递过来的信号,大脑做出指示,然后再一次通过神经突触的协助,完成后续动作。
突触囊泡循环是突触事件中最核心的部分,突触囊泡通过胞吐和内吞作用完成一次突触囊泡循环。当突触囊泡膜与突触前膜融合时, 递质释放, 即为胞吐; 囊泡膜与质膜分离重新内陷,称为胞吞。突触囊泡循环主要包括以下九个步骤:锚靠、激活、融合/出胞、入胞、移位、内质体融合、出芽、神经递质摄取、囊泡移回突触前膜的活化区。
与经典神经-肌肉接头突触和中枢神经元常型突触相比较,还存在一种特殊的神经突触——缎带突触或称带状突触(ribbon synapse)。带状突触被发现存在于视网膜的感光细胞和双极细胞,以及内耳前庭毛细胞和耳蜗内毛细胞。视网膜及内耳的带状突触通过紧张性释放神经递质传导不同强度的光和声音信息。
与中枢神经系统突触的胞吐机制相似,带状突触囊泡释放也分为两种:快速释放池(readily releasable pool,RRP)和慢速释放池(slowly releasable pool,SRP),但是带状突触递质释放较经典海马神经元突触囊泡释放速率明显增快。
神经突触传递是研究神经信息处理、传递的神经生物学的中心问题。艾美捷科技作为专业的生命科学领域解决方案供应商,为您推荐Synaptic Systems品牌的可用于突触功能研究相关的产品集合。这些产品包含了抗体、血清、上清液等等,可用于许多应用,包括ELISA,免疫印迹(WB),免疫组织组化(IHC),免疫细胞化学(ICC)和免疫沉淀(IP)等,部分产品还可以用于EM,IHC-P/FFPE等。
中文名 | 英文名 | 货号 | 应用类型 | 规格 |
单克隆小鼠纯化IgG抗体 | Monoclonal mouse purified IgG | 106 011 | WB,IP,ICC,IHC,IHC-P/FFPE,EM,ELISA | 100 ug |
Monoclonal mouse purified IgG | 105 011 | WB,IP,ICC,IHC,IHC-P/FFPE,EM,ELISA | 100 ug | |
Monoclonal mouse purified IgG | 311 011 | WB,IP,ICC,IHC,IHC-P/FFPE | 100 ug | |
Monoclonal mouse purified IgG | 202 111 | WB,IP,ICC,IHC,IHC-P/FFPE,ELISA | 100 ug | |
Monoclonal mouse purified IgG | 201 011 | WB,IP,ICC,IHC,IHC-P/FFPE,ELISA | 100 ug | |
单克隆小鼠杂交瘤上清液 | Monoclonal mouse hybridoma supernatant | 147 021 | WB,IP,ICC,IHC,IHC-P/FFPE,EM | 300 ug |
多克隆兔纯化抗体 | Polyclonal rabbit purified antibody | 104 103 | WB,IP,ICC,IHC,IHC-P/FFPE,EM | 50 ug |
Polyclonal rabbit purified antibody | 272 003 | WB,IP,ICC,IHC,IHC-P/FFPE | 50 ug | |
Polyclonal rabbit purified antibody | 135 403 | WB,IP,ICC,IHC,IHC-P/FFPE,EM,ELISA | 50 ug | |
Polyclonal rabbit purified antibody | 257 003 | WB,IP,ICC,IHC,IHC-P/FFPE | 50 ug | |
Polyclonal rabbit purified antibody | 224 003 | WB,IP,ICC,IHC,IHC-P/FFPE,EM | 50 ug | |
多克隆豚鼠血清 | Polyclonal Guinea pig antiserum | 135 404 | WB,IP,ICC,IHC,IHC-P/FFPE,EM | 100 ul |
Polyclonal Guinea pig antiserum | 101 004 | WB,IP,ICC,IHC,IHC-P/FFPE | 100 ul |
参考文献(部分):
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FWThe Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
2.Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells.
Fukushima H, Yoshioka M, Kawatou M, López-Dávila V, Takeda M, Kanda Y, Sekino Y, Yoshida Y, Yamashita JK
PloS one (2020) 1511: e0241287. 311 011 ICC; tested species: human
3.Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.
Halloin C, Schwanke K, L?bel W, Franke A, Szepes M, Biswanath S, Wunderlich S, Merkert S, Weber N, Osten F, de la Roche J, et al.
Stem cell reports (2019) 132: 366-379. 311 011 ICC, FACS; tested species: human
4.Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess.
Ting S, Lam A, Tong G, Chen A, Wei H, Wu J, Lam YN, Reuveny S, Oh S
Stem cell research (2018) 31: 161-173. 311 011 IHC; tested species: human
5.Rat atrial engineered heart tissue: a new in vitro model to study atrial biology.
Krause J, L?ser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T, Stenzig J
Basic research in cardiology (2018) 1135: 41. 311 011 IHC-P; tested species: rat
6.Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, Christalla P, Malan D, Hesse M, Bosio A, Fleischmann BK, et al.
PloS one (2015) 1011: e0143538. 311 011 FACS
7.Cadherins mediate cocaine-induced synaptic plasticity and behavioral conditioning.
Mills F, Globa AK, Liu S, Cowan CM, Mobasser M, Phillips AG, Borgland SL, Bamji SX
Nature neuroscience (2017) 204: 540-549. 135 404 EM; tested species: mouse
8.Satb2Cre/+ mouse as a tool to investigate cell fate determination in the developing neocortex.
Ambrozkiewicz MC, Bessa P, Salazar-Lázaro A, Salina V, Tarabykin V
Journal of neuroscience methods (2017) 291: 113-121. 257 003 IHC; tested species: mouse
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