【相关文章】
自由基与多种人类疾病的发生发展都有着密切的关系。在清除自由基的过程中,抗氧化剂发挥着重要的作用。因此,了解抗氧化剂的作用及能力就变得至关重要。
在评价抗氧化剂能力的诸多方法中,抗氧化能力ORAC(又称,氧自由基吸收能力,Oxygen Radical Absorbance Capacity)分析法,被认定为检测抗氧化剂抗氧化能力的标准方法,是目前普遍被接受的一个标准评价手段。由于该法具有接近机体生理条件、操作简单、认可度和灵敏度高、准确性和重现性佳、高通量、不易受人为因素干扰等优点,被广泛用于农业,食品,保健品,药品及医学领域。
那么如何分析样本中氧自由基抗氧化能力/氧自由基吸收能力(ORAC)?作为专业的生命科学医药原料供应商,艾美捷科技为您推荐:众多文章引用的,氧自由基抗氧化能力(ORAC)活性检测试剂盒
产品 | 氧自由基抗氧化能力(ORAC)检测试剂盒 OxiSelect ORAC Activity Assay Kit |
货号 | CBL-STA-345 |
检测方法 | 荧光法 |
适用样本类型 | 血清/血浆,尿液,细胞裂解液/提取物,组织裂解物,营养样品(食品,植物等等) |
说明书下载 | 点击下载 (请于实验前,详细阅读说明书) |
试剂盒原理 | ORAC分析法中的自由基主要来源于偶氮化合物2,2'-偶氮-双-(2-眯基丙烷)氯化二氢(2,2'-azobis(2-amidinopropane)dihydrochloride,AAPH)热分解产生的过氧化氢自由基,也可以是芬顿(Fenton)反应过程中产生的羟自由基,以荧光素钠(Sodium Flourescein,FL)为荧光探针,观察自由基与荧光探针作用后,探针荧光强度的衰退过程,以水溶性维生素E类似物(Trolox)作为抗氧化标准物质,检测体系中各种抗氧化剂延缓探针荧光强度衰退的能力,以此评价抗氧化剂的抗氧化能力。 |
实验优势 | 1 简单快捷:不到2小时完成实验; 2 样本适用:多样性 3 提供标准品; |
试剂盒组分 | 1. 96-well Microtiter Plate 2. Fluorescein Probe (100X) 3. Free Radical Initiator 4. Antioxidant Standard (Trolox) 5. Assay Diluent (4X) |
保存条件 | 收到后,Fluorescent Probe和Antioxidant Standard (Trolox) 保存于-20℃;其它所有试剂组分保存在4℃。 |
* 本产品仅适用于科研用途.
氧自由基抗氧化能力(ORAC)活性检测试剂盒,原理与标曲展示:
氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图
氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图
FAQ:
1.是否可以测试冷冻保存的样品?
RE:通常适用于在 -80℃ 下保存两个月以内样品,但我们建议尽可能测试新鲜样品以获得最佳结果
2.ORAC、HORAC和TAC检测之间的区别,如何选择?
RE:所有三种测定都测量抗氧化能力,但略有不同。ORAC检测分析氧自由基的抗氧化能力;HORAC检测分析羟基自由基的抗氧化能力;TAC基于单电子转移(SET)机制和铜离子,检测总体抗氧化能力。总体而言,研究人员更偏好选择ORAC分析而不是HORAC,因为过氧自由基似乎比羟基自由基更相关。
3.更多常见问题,请点击:cellbiolabs.com/faq/oxidative-stress-faq/orac-assay
近期发表文章(仅摘取部分展示):
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