NAD/NADH比率检测试剂盒，比色法荧光法简单快速！

       NADH，烟酰胺腺嘌呤二核苷酸，还原态，还原型辅酶Ⅰ。N指烟酰胺，A指腺嘌呤，D是二核苷酸。葡萄糖代谢时直接经代谢所产生的ATP是十分的少的，而代谢产生的NADH或FADH2经由一个电子传递与氧化磷酸反应可产生大量的ATP。NADH分子是线粒体中能量产生链中的控制标志物。NADH水平的上升指示代谢失衡的出现。

 

 

       监视NAD/NADH的氧化还原状态是表征活体内线粒体功能的最佳参数。紫外光可以在线粒体中激发NADH产生荧光，用来监测线粒体功能。一般来说，涉及到氧化还原的反应都用得到，比如呼吸作用，光合作用，等等氨会抑制呼吸过程中的电子传递系统，尤其是NADH。因此NAD/NADH水平的监控是能量传递、细胞核组织氧化还原态等研究的主要兴趣点。

 

       有没有一款可以同时检测，NAD+,NADH，以及NAD+/NADH比率，这三个指标的试剂盒呢？

 

        艾美捷科技特向您推荐，已被广泛使用，发布文章不计其数的高品质NAD/NADH比率检测试剂盒。

 

 

       *样品通过NAD萃取，同时检测（NAD+NADH）与（NAD）的结果，相减计算得出NADH含量，以及NAD/NADH的比率。  

 

 

       原理：Amplite 比色法NAD/NADH比率检测试剂盒，提供一个比色法原理的检测培养细胞的总NAD/NADH，和NAD/NADH ratio的方法。在这个实验中，裂解液中NAD可以被NAD萃取液提取，通过酶反应被转化为NADH，然后利用NADH探针反应，产生黄色染料，此染料可以在460nm处测吸收峰，或者加入增强剂后，可以检测635nm吸收峰。染料产生颜色与细胞裂解液中NAD 或者NADH的浓度呈正比。  

 

 

《卓越的标准曲线》

 

《发表文章结果：Ren, T., et al. Oncogene  36.42(2017).》

   

 

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• MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells

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• Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase

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• Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway

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• Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio

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• A Snapshot of the Plant Glycated Proteome STRUCTURAL, FUNCTIONAL, AND MECHANISTIC ASPECTS

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• AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD+ elevation

Authors: Xiaojuan Han, Haoran Tai, Xiaobo Wang, Zhe Wang, Jiao Zhou, Xiawei Wei, Yi Ding, Hui Gong, Chunfen Mo, Jie Zhang Journal: Aging cell (2016): 416—427

 

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